Analysing Hi-C data with Galaxy Australia

Dr Tristan Reynolds (Melbourne Bioinformatics, The University of Melbourne)

The three-dimensional organisation of the genome plays a crucial role in regulating gene expression and cellular processes. Hi-C (High-throughput Chromosome Conformation Capture) is a powerful technique that captures long-range chromatin interactions, enabling the identification of topologically associating domains (TADs) and chromatin loops. Hi-C data analysis involves computational methods for processing, normalising, and visualising these datasets to unravel the relationships between genome architecture and gene regulation.

During the workshop attendees can expect to gain an understanding of approaches to and practical experience in: mapping Hi-C reads to a reference genome and using HiCExplorer to create and visualise a Hi-C contact matrix and investigate TADs and chromatin loops.

This training workshop will be conducted using Galaxy Australia and the workshop materials are based on the following Galaxy Training Network tutorial:

• Hi-C analysis of Drosophila melanogaster cells using HiCExplorer

It is important that you are familiar with navigating the Galaxy platform as the workshop will not include introductory content on how to use the platform.

By the end of this training, participants will be able to:

• Understand why Hi-C analysis is useful
• Identify what is ‘chromosome conformation capture’
• Perform the main steps to generate and plot a Hi-C contact matrix

This Free workshop is available to staff and students at The University of Melbourne and its affiliated institutes.

You must register for this event using an affiliated institutional email address or your registration may be cancelled.

Researchers who have, or will soon acquire, Hi-C data that they need to analyse themselves. No programming experience is required.

This is an in person hands-on workshop and attendees must bring their own computers (laptop chargers also recommended). No software needs to be installed for this workshop. The following is required:

• Access to internet via uniwireless or Eduroam
• Web browser (Firefox or Chrome recommended)
• Participants should register for a Galaxy Australia account using their institutional email address

If you require any further information, or have any access requirements in order to participate in this workshop, please contact us as soon as possible to discuss your requirements:

We recommend that following our eventbrite page if you wish to be alerted when we release new workshops for registrations. If you require any further information, please contact Melbourne Bioinformatics at: YmlvaW5mb3JtYXRpY3MtdHJhaW5pbmcgfCB1bmltZWxiICEgZWR1ICEgYXU=

Starting 1 August 2025, participants who miss two or more workshops without proper notice (less than 48 hours or attending under 50%) within 12 months will be placed on a 6-month waitlist for full workshops. To avoid being marked as a no-show, cancellations must be made at least 24 hours in advance, with exceptions allowed for emergencies.Please read the full policy here: https://mbite.org/guides/policy/

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