The CRL Molecular Imaging Center (MIC) is a shared light microscopy core at the University of California, Berkeley, specializing in state-of-the-art laser-based fluorescence techniques. The MIC offers training and expertise in 20 different microscope systems, including live cell and in vivo imaging, laser scanning (LSM) and spinning disk (SDC) confocal, multi-photon (2p), fluorescent lifetime imaging (FLIM), light-sheet microscopy (SPIM), super resolution (Airyscan), slide scanning and patterned illumination for optogenetic manipulation and readout. The MIC provides offline computer analysis workstations for image processing, visualization and analysis, including GPU workstations. The MIC is accessible to all researchers across the UC Berkeley campus, as well as non-UCB, and non-academic laboratories and companies.