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3 Days National Level Workshop “NGS Data Analysis: RNA & ChIPseq


3 Days National Level Workshop “NGS Data Analysis: RNA & ChIPseq

Our 3 day comprehensive workshop on “NGS Data Analysis: RNA seq and ChIP seq Analysis" aims at providing systematic Hands-on-Training on using advanced Data Analysis application/tools. This workshop had been conceptualized by eminent scientist having substantial experience in the field of Data Analysis.
Salient Features
 Introduction to Galaxy platform and walk through a multi-step next generation sequencing (NGS) data analysis, starting with quality control.
 Review common choices in NGS data analysis, and demonstrate them within the context of Galaxy, taking full advantage of Galaxy's rich tool set and visualization capabilities.
 Brief overview of what is needed to set up your own local Galaxy instance. Galaxy is an open-source, NIH-funded project.
Topics Covered
• Introduction to sequencing technologies from a data analysts view
• Common NGS data analysis issues
• Applications of sequencing technologies
• Introduction to file formats (e.g. FASTQ, VCF, SAM, BAM) and databases (e.g. SRA, GEO, NCBI)
• Pre processing of raw reads: quality control (FastQC), adapter clipping, quality trimming
• Introduction to read mapping (Alignment methods, Mapping heuristics)
• Read mapping (BWA, Bowtie2, STAR)
• Usage of important NGS toolkits (samtools, BEDtools)
• Mapping statistics
• Visualization of mapped reads (IGV, UCSC)
• Understanding the transcriptome data and how its genenrated
• Analyzing the transcriptomic data to understand gene expression and differential gene expression
• Introduction to ChIP-seq analysis with Galaxy

Practical Application:
• Understand the Next generation sequencing
• Discuss the advantages and limitations of the sequencing technologies
• Learn how to use Galaxy server to analyze the NGS data
• Deal with the raw Illumina data with quality evaluation and filtering
• Map reads to the reference genomes, call SNPs and predict their phenotype consequence
• Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data
• Use some bioinformatics software and tools to aid with the analysis of NGS data
• Understanding Gene expression and Differential gene expression
• Visualize your data in genome browsers and make conclusions
• Participants will receive workshop training certificate.

Databases and Tools & Software’s used
NCBI: PubMED, MeSH, Gene Database, GeneMark, GenScan, BLAST, Galaxy, BWA, Freebayes, wAnnovar, RNA-Star, HTseq2, DESEQ, Deep Tool and many more

Eligibility: Working professional,Scientists /Research fellows (National Laboratories, Universities & other R&D institutions) and Graduate / Post graduate students in Engineering / Biological / Chemical sciences/ Medical discipline.

Batch and Deadline for Registrations and Fee

Type Dates Student Researcher/ NRI/
Professor Foreigner

Early-bird: Till 18th February 2019 INR 3000/- INR 3500/- INR 4000/-
Standard: 19st Feb 2019 to 2nd March 2019 INR 3500/- INR 4000/- INR 4500/-
On the Spot: 3rd to 7th March 2019 INR 4000/- INR 4500/- INR 5000/-

*Includes lunch , & snacks.
**Id proof required at the time of registration.
***Fees are non transferrable & non refundable in any case.

Mr.Sameer Chaudhary
CSO, RASA Life Science Informatics.

Sakshi Rawat
Application Scientist, RASA Life Science Informatics.

Venue: RASA Life Science Informatics
4th floor.46/10 shakuntal,
Law college Road,Erandwane 411004
Phone No. 7875793891/ 7038770985/7798668315

Bank Name & Branch: IDBI, F.C. Road, Pune.
A/C Number: 007102000025762
IFSC Code: IBKL0000007

Map RASA Life Science Trainings, 4th floor.46/10 Shakuntal, Law college Road,Erandwane Phone No. 020-65600408, Pune, Maharashtra 411004, Pune, India
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RASA Life Science Informatics