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Genome Editing with CRISPR-Cas9

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Genome Editing with CRISPR-Cas9


Class in four sessions:

February 19, 20, 21, and 28

6:00 PM - 10:00 PM 

CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes and increasing its accessibility.  Want to learn how to do hands-on genome editing? This is an intensive laboratory class with limited space. Class size is limited to ten students.

In this class you will:



Learn how to culture and work with Saccharomyces cerevisciae(brewers yeast)



Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion



Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome



Use the constructs you produce to transform yeast and confirm the results by DNA sequence analysis



Prerequisites:



You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes and PCR (Genspace Biotech Crash Course, Biohacker Boot Camp or equivalent lab experience)





Will Shindel studied Biology at Arcadia University, where he investigated the role of insulator elements in genome organization. After graduating, he spent several years working in the pharmaceutical industry.  It was during this time that he discovered DIY biology and first came to Genspace. Will has worked on several of Genspace’s winning iGEM teams, was for many years our lab manager and science mentor, and is a co-founder at Brooklyn Bio.



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